Responses
Contents
Responses¶
For all prompts, make sure to include all components that are required. For any of the prompts, you can of course also include anything else that you noticed or wondered that adds to the report of your results.
Your Name:
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Experiment 1: Strength-Duration Threshold¶
Prompt 1 (10 points):
*Create a Figure with the following panels:
A. A strength-duration scatterplot for the MGF with a hyperbolic fit line. Rheobase, 2* rheobase, and chronaxie need to all be annotated on the plot.
B. A strength-duration scatterplot for the LGF with a hyperbolic fit line. Rheobase, 2*rheobase, and chronaxie need to all be annotated on the plot.
C. Line plot of the strength-duration hyperbolic fit function of both MGF and LGF on the same plot. Chronaxie need to be annotated on the fit function for each neuron.
D. (i,ii,iii) Example data showing the spiking response to 2 msec duration, chronaxie duration, and minimum effective stimulus duration.
Write a caption to the figure that includes:
-a description (ie. legend) for each figure panel including all information needed to assess your reported results
-the rheobase and chronaxie for the MGF and LFG
-an interpretation of the results in terms of neural excitability
-hypothesize about the anatomical/biophysical reason for the MGF compared to the LGF results (will depend on whether you found they were different or the same)
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Experiment 2: Paired Pulse Stimulation¶
Prompt 2 (10 points):
Create a figure with the following panels:
A. Scatterplot of action-potential amplitude against paired pulse delay (inter-pulse interval) in response to each of the two stimulus pulses on each trial (the first and second spike amplitudes should be plotted on the same plot in different colors).
B. Example data showing the spiking response at: above the refractory period, within the refractory period, and below the refractory period.
Write a caption to the figure that includes:
-a description (ie. legend) for each figure panel including all information needed to assess your reported results
-The relative refractory period range
-The absolute refractory period range
-Describe (and interpret) the experimental results (action potential from the second pulses) with reference to the control data (action potential from the first pulses)
-A mechanistic explanation for the absolute and relative refractory periods
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Prompt 3 (10 points):
Create a figure with the following panels:
A. Example data showing the spiking response to a paired pulse with annotation of all measurements used in Panel B (all x and y axis variables).
B. Scatterplot of action-potential conduction velocity against paired pulse delay (inter-pulse interval) in response to each stimulus pulse on each trial (data for the first and second spikes should be plotted on the same plot in different colors).
Write a caption to the figure that includes:
-a description (ie. legend) for each figure panel including all information needed to assess your reported results
-An interpretation of the results
-Describe the experimental results (action potential from the second pulses) with reference to the control data (action potentials from the first pulses).
-A comparison of these results with your results from the comparable experiment in the conduction velocity lab (note which Giant Fibers you measured in each experiment and how you know which one it is in each)
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